Detection of telomerase activity by combination of TRAP method and scintillation proximity assay (SPA)
نویسندگان
چکیده
منابع مشابه
Detection of telomerase activity by combination of TRAP method and scintillation proximity assay (SPA).
Numerous studies on telomerase, an enzyme which can elongate telomere ends (1,2), led to the increasing evidence that the presence of telomerase in cells that normally lack it may contribute to the uncontrolled cell growth of cancer (3–6). Those findings, together with the fact that the enzyme is not active in normal cells (7), make it an interesting target for cancer drugs, as well as an impor...
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Abstract Background and Objective: Breast cancer is the most common cancer in women. Telomerase enzyme is one of the major factors causing the development and proliferation of immortal cells such as cancer cell. The aim of this study was to evaluate telomerase activity in breast tissues of breast cancer patients and healthy people. Material and Methods: In this descriptive study, the sampl...
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G protein-coupled receptors (GPCRs) represent attractive drug targets because they participate in a wide range of physiological processes and are believed to play a role in a variety of disease states. At least a third of all prescription drugs on the market today are molecules acting as agonists or antagonists of the GPCR. Receptor-ligand interaction studies are pivotal in the successful ident...
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introduction: telomeric repeat amplification protocol assay (trap assay) is a standard established technique to detect the activity (expression) of telomerase in the cancerous samples. it includes a time-consuming procedure that has significant drawbacks such as high cost, complicacy, time-consuming procedure, equipment-dependent steps and false-positive signals caused by inclusion of pcr insid...
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We describe a novel, immunospecific scintillation proximity assay for determining cholesteryl ester transfer protein (CETP) activity in total human serum and in reconstituted experimental mixtures. The assay is based on the measurement of radiolabeled cholesteryl esters transferred from a tracer dose of biosynthetically labeled high-density lipoprotein subfraction 3 to unlabeled apolipoprotein ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 1362-4962
DOI: 10.1093/nar/24.6.1175